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KMID : 0379119880160020110
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Korean Journal of Mycology
1988 Volume.16 No. 2 p.110 ~ p.110
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Application of Calorimetry to Mycology
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Beezer, A. E.
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Abstract
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Changes in heat content or enthalpy are the universal accompaniment of all reactions both chemical and physical. Thus since metabolism of microbial species can be regarded as a complex sequence and conjunction of reactions then the process and progress of metabolism can be followed by monitoring these changes in energy output.
The growth of Saccharomyces cerevisiae can be followed, therefore in defined medium growing on a single carbon source e.g. glucose. The power-time curve observed(in a flow microcalorimeter) will therefore show an exponential phase followed by a rapid decline on exhaustion of, say, glucose. More complex media can be studied and the sequence of substrate utilization discovered-together with the kinetic parameters associated with each process.
Extension of these studies to the following topics will be explored:
(a) identification of microbial species
(b) metabolism on a single substrate(i.e. no growth)
(c) the interaction of antifungals with Saccharomyces cerevisiae and Candida albicans.
The bioassay procedures described in (c) are rapid(20min), sensitive(e.g. 0.1 unit ㎝^(-3) of nystatin detectable) and reproducible(+1.3%). The parameters are much better than those derived from plates or tubes. Examples of synergic and antagonistic interactions between antibiotics given in combination will be discussed. Extension of these studies to Quantitative Structure Activity Relationships will also be reported.
The bioassay procedures described in the literature depend, in part, on the use of liquid nitrogen stored cultures. Such cultures have maintained constant properties over a period in excess of 6 years and thus raise the possibility that constant performance(from the microbe) can be used to evaluate the capacity of complex media e.g. molasses, to maintain adequate/good growth on a commercial scale. Examples of the identification of $quot;good$quot; and $quot;poor$quot; commercial molasses will be given.
Further discussion will relate to the study of synchronous cultures and to the cell cycle.
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